Next – Generation Sequencing

LAB
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  • 10 week duration

PCR Duplicates and Statistical approach

Remove PCR Duplicates

We need to assess how many of the mapped reads originated from the same RNA molecule (PCRduplicates) before calculating the expression levels. Computationally, read duplicates are defined by
their mapping position, reads with same mapping position and length are considered as duplicates.
There is no clear guideline on removing or retaining PCR duplicates [19], however if the PCR
duplicates constitutes major fraction of the data, it is always good to compare the results with and
without duplicates.