Next - Generation Sequencing

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LAB

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Free

27 lessons
0 quizzes
10 week duration

NGS DNA sequencing strategies presently beneath development embrace reading the sequence as a
DNA strand transits through solid-state nanopore and microscopy-based techniques, like atomic
force research or TEM strategies.

Overview
Module 1
- Quality Check using fastqc and Multiqc
- Practice
Module 2
- Removal of rRNA and Adapter Trimming
- Practice
Module 3
- Indexing and Alignment process
- Practice
Module 4
- PCR Duplicates and Statistical approach
- Practice
Module 5
- Gene expression using featureCounts
- Practice
Module 6
- Gene Expression Analysis using DESeq2
- Practice
Module 7
- Introduction to R Programming
- Practice
Module 8
- Various Packages using R for Gene Expression
Module 9
- Gene Ontology and pathway Analysis
- Practice
RNASeq Pipeline using edgeR
- Complete Pipeline

LAB

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Next – Generation Sequencing

Overview

Module 1

Module 2

Module 3

Module 4

Module 5

Module 6

Module 7

Module 8

Module 9

RNASeq Pipeline using edgeR

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