How Viruses Work: Virology

A general approach for purifying a virus from tissue culture cells

1. Infect the Virus to a confluent cell culture monolayer
2. Incubated the infected cells at 37°C at 24-48 hrs
3. Harvest the cells and its supernatant
4. Freeze-thaw couple of time to release the viral particle from the cells
5. Ultracentrifuge at high speed under sucrose cushion or gradient
6. ollect the viral pellet
7. Check for its purity and Quantification