Coronavirus disease (COVID-19) outbreak

Replication of Novel Coronavirus or SARS-CoV-19

The next process for the virus survival is its RNA replication and it is the most critical step done by the virus for its survival inside the host cells. ORFs or Open Reading Frame are used in this process of replication i.e. two polyproteins & a slippery sequence. Nsp proteins are present in the polyproteins which have a major role in replication but also attack the immune system of the host during viral replication. The mechanism of replication of COVID-19 is very similar to the SARS-CoV. In replication, the genomic RNA contains a 5’end region that has the untranslated regions with the TRS (transcription regulation sequence) present at the below region of the genome. During the replication process inside the host, the Nucleocapsid protein of the virus attach to the genome while the Membrane protein is associated with the endoplasmic reticulum. Moreover, Nsp proteins assembled with the RNA into helical twisted anatomy and buds into the Endoplasmic reticulum lumen. These mechanisms were discovered in the foregoing viruses and may have a significant role in SARA-CoV-2.  In the replication process, we evident that the intending Nsp proteins might enable humans to develop a strategy that will be unable to continue the viral infection.