Concept of Column chromatography

Mechanism of Column Chromatography

According to the column chromatography mechanism, two different phases involved in the column chromatography mechanism.

• Stationary Phase – it is defined as a solid material which must have better adsorption capability and meets the standard criteria such as:

– Stability and inertness of the particles

– should be colorless, readily, and inexpensive

– uniform shape and size of the particle

– should be perfect for separation of complex mixtures of different compounds

– must allow the free movement of the mobile phase.

• Mobile Phase – this is nothing but made up of solvents and have some criteria such as:

– Sample or sample compound can be subjected into the column

– should act as a developing agent or helps in the movement of components

– should act as an eluting agent.