Bioinformatics Tools Training Program

Notes: I. Output of each submission must be captured with snipping tool.
II. write short interpretation along with the screenshot.

Q. 1. Retrieve the FASTA Sequence of Leptinotarsa decemlineata Egfr from Biological Database
and find out the Similarity using BLAST. Write the Result and Interpretation.
Q. 2. Retrieve the FASTA sequence of EGFR gene of two different organism. 1) Perform Global
Pairwise Alignment using EMBOSS NEEDLE. 2) Perform Local Pairwise Alignment using
EMBOSS WATER.
Write Interpretation in Brief.
Q. 3. Obtain the Multiple Sequence Alignment of Bacillus megaterum, Cupriavidus necator,
Aeromonas hydrophila. Ii) Construction of Phylogenetic tree.
Q. 4. Design the primer of BRCA1 gene using primer3plus. Interpret the Results.
Q. 5. Retrieve the FASTA Sequence of the BRCA1 gene – 1) determine the restriction enzyme site
of the enzymes using NEB Cutter ii) Generate a custom digest map and a virtual gel of methylated
and unmethylated DNA